Fatal intoxication with U-47700 in combination with other NPS (N-ethylhexedrone, adinazolam, 4-CIC, 4-CMC) confirmed by identification and quantification in autopsy specimens and evidences

Abstract Purpose We present a case of fatal intoxication with U-47700 in combination other NPS ( N -ethylhexedrone, adinazolam, 4-chloro- -isopropylcathinone (4-CIC), 4-chloromethcathinone (4-CMC) and sertraline) confirmed by identification quantification biological materials evidences found at the scene 2017 Poland. Methods Blood urine samples were extracted ethyl acetate from alkaline medium (pH 9); powders/crystals diluted methanol. The analysis was carried out using ultra-high-performance liquid chromatography–tandem mass spectrometry. Validation criteria evaluated for blood concentrations 10 100 ng/mL. Results validation parameters method within acceptable ranges. In presented case, determined drugs as follows, blood: U-47700, 1470 ng/mL; 58 18 4-CIC, 8.0 4-CMC, 1.7 urine: 3940 147 82 130 417 Sertraline (blood, 89 urine, 32 ng/mL) also both materials. same substances 5 powders/crystals: (12% weight), -ethylhexedrone (54%), adinazolam (14%), 4-CIC (23%), 4-CMC (26%). After 775 days storage, + 4 °C, most stable substance sertraline less, synthetic cathinones, especially 4-CMC. Conclusions described postmortem first time literature. paper showed stability study these stored °C days.